The Spleen And Anaemia Experimental And Clinical Studies

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By acid alcohol and zinc acetate being taken into account. Instead of using tap- water for dilution of the final extract, 60 per cent, alcohol was required to avoid the development of a precipitate. " Wilbur and Addis found that, while urobilin is a nor- mal constituent of the urine, the amount is so small that ordinarily it can be demonstrated only by extracting large quantities of urine. A positive result, therefore, in twenty- four-hour specimens indicates an abnormal increase over the amoun...ts usually present. In the stools of ten adults with supposedly normal haemoglobin metabolism they found that the average daily excretion of urobilin varied between 286 THE SPLEEN AND ANAEMIA 3307 and 8737 dilution values, with a general average of 6475. It is important, however, to note that, on account of the wide daily fluctuations, examinations of single twenty-four-hour specimens are of little value. So long as stools were kept from exposure to light there did not seem to be a very rapid loss of urobilin, and, as the desired figures represent the combined value of urobilinogen and urobilin, the amount of the former that changes to the latter while standing is immaterial.

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