The Synthesis And Characterization of Reversed Phase Stationary Phases for High Performance Liquid Chromatography

Cover The Synthesis And Characterization of Reversed Phase Stationary Phases for High Performance Liquid Chromatography
The Synthesis And Characterization of Reversed Phase Stationary Phases for High Performance Liquid Chromatography
Barnes, Karen Wink
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Pryde and Darby (87) bonded gamma- aminopropyl trie thoxy si lane to spherical alumina and used the material for the analysis of paraquat and diquat. These phases, while useful for the researcher's particular applica- tion, would not be useful to a large number of chromatogra- phers. Hirata and Novotny (98) attempted to prepare a non- polar RP column by reacting, in situ, octadecylchlorosilane with alumina particles contained within a micr ocap i 1 lar y column and used the column for RP experi...ments. The column became more polar with use as the bonded material was cleaved.
Laurent and coworkers (89-91), finding similar difficulties, reported that hexame thy 1 d isi lazane (HMDS) bound to alumina was readily displaced by MeOH and H .-, . This phenomenon was 103 repeated with other reagents including trimethylchlorosilane and tertiary amines. They postulated that the lability of the material in polar solvents is due to the ionic nature of the Al-0 bond which makes the material more susceptible to nucleophilic attack than the less polar analogous Si-0 bond.


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