The Technical Control of Dairy Products; a Treatise On the Testing, Analyzing, Standardizing And the Manufacture of Dairy Products

Cover The Technical Control of Dairy Products; a Treatise On the Testing, Analyzing, Standardizing And the Manufacture of Dairy Products
The Technical Control of Dairy Products; a Treatise On the Testing, Analyzing, Standardizing And the Manufacture of Dairy Products
Mojonnier, Timothy, 1876-
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All glassware and all apparatus such as kettles, funnels and filtration flasks, must be kept scrupulously clean by running hot water over or through them after use before the agar has had time to harden. There is danger otherwise of dried particles of agar chipping oflf and giving rise to sediment in future batches of agar which in the poured plates may be mistaken for colonies.
Procedure No. 1. Mix all of the ingredients together cold.
Heat in an autoclave at 15 lbs. pressure for 40 to 90 minu
...tes according to the quantity of medium being made in each batch.
Allow the autoclave to cool very slowly so as not to disturb the sediment. Decant through a cotton filter taking care not to pour the sediments on the cotton until the bulk of the liquid has passed through.
This simple procedure with certain brands of peptone and grades of agar gives excellent results.
Procedure No. 2. Where large quantities of agar are to be prepared the following procedure has been found useful. Pre- pare two separate solutions: Mixture A.


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