Transactions

Cover Transactions
Transactions
Tyron, James, 1841- [from Old Catalog] Ed
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Lond., July 26, 1904, p. 154) found it necessary to keep the suspension of bacteria in the incubator twenty-four hours after heating at 60° C.
for thirty minutes. The suspension was then cultured and at the same time enumerated; 25,000,000 to 75,000,000 bacteria were used.
Should the culture media show evidence of bacterial growth, the technique was repeated until the organisms had lost their power of further multipHcation.
E. H. Schordor, of Rockefeller Institute, makes a reference to the use
...of killed cultures of streptococci, employing 25,000,000 to 100,000,000. Dr. Simon, of Baltimore (Exp, Med,, September 21, 1907), in a paper read before the Association of American Physicians last assembled in Washington, D. C, makes the most remarkable statement that he can see no difference in the reaction of a patient to his vaccine whether 25,000,000 or 1,000,000,000,000 staphylococci are given. And Wright and Reed, when employing the colon bacillus for immunization in cases of cystitis, make use of a sterilized vaccine containing 200,000,000 organisms.

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