Translation of Potyvirus Rna in a Rabbit Reticulocyte Lysate
Translation of Potyvirus Rna in a Rabbit Reticulocyte Lysate
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Coupled 52 with an immunoabsorbent {-i.e., Staphylococcus aureus Cowan strain 1), this type of product analysis is rapid and definitive. The assay is limited, however, by the availability of antisera to viral products.
Because of the various inclusions formed in vivo with potyvirus infections ; and the availability of inclusion and capsid protein antisera, the genetic analysis of the potyvirus genome using immunoprecipi tat ion has been undertaken.
The synthesis of potyvirus cell -free products... was affected by the RNA quality. Potyvirus RNA isolated in the presence of Proteinase K yielded a higher percentage of intact 39 S RNA, as determined on linear-log sucrose gradients. This RNA stimulated the incorporation of 35 [ S] methionine into trichloroacetic acid (TCA) precipitated products 50 to 60 times over endogenous levels. Potyvirus RNA isolated without Proteinase K typically stimulated the cell-free system 15 to 20 times over endogenous levels. In vitro products analyzed by SDS-PAGE, varied depending on the RNA used.
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To quickly assess the difficulty of the text, read a short excerpt:
Coupled 52 with an immunoabsorbent {-i.e., Staphylococcus aureus Cowan strain 1), this type of product analysis is rapid and definitive. The assay is limited, however, by the availability of antisera to viral products.
Because of the various inclusions formed in vivo with potyvirus infections ; and the availability of inclusion and capsid protein antisera, the genetic analysis of the potyvirus genome using immunoprecipi tat ion has been undertaken.
The synthesis of potyvirus cell -free products... was affected by the RNA quality. Potyvirus RNA isolated in the presence of Proteinase K yielded a higher percentage of intact 39 S RNA, as determined on linear-log sucrose gradients. This RNA stimulated the incorporation of 35 [ S] methionine into trichloroacetic acid (TCA) precipitated products 50 to 60 times over endogenous levels. Potyvirus RNA isolated without Proteinase K typically stimulated the cell-free system 15 to 20 times over endogenous levels. In vitro products analyzed by SDS-PAGE, varied depending on the RNA used.
What to read after Translation of Potyvirus Rna in a Rabbit Reticulocyte Lysate?
You can find similar books in the "Read Also" column, or choose other free books by Dougherty, William George to read onlineMoreLess
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