Trypanosoma Americanum, a Common Blood Parasite of American Cattle

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445 do.
5 532 1910 Apr. 7 533 550 Apr. 13 551 .do.
552 do.
5.53 ...do ' Culture in incubator.
» 1 drop=0.067o n. c.
20 TRYPANOSOMA AMERICANUM, A BLOOD PARASITE OF CATTLE.
Results obtained with measured quantities of blood — Continued.
Culture.
674.
675.
576.
577.
618.
622.
623.
624.
625.
Date.
1910 Apr. 21 ..do ..do ..do July 14 .do.
.do.
.do.
.do.
Cow.
697 697 697 697 697 697 697 697 697 Quantity of blood.
Drops.
5 5 10 10 5 c. c.
1.0 1.0 1.0 1.0 Result ( + , positive; — , negative).
Days.
Her
...e it is seen that the smallest quantit}^ of blood to give a positive result was 5 drops, or 0.3375 c. c, and that this result was obtained only once out of three trials. Assuming 6,000,000 red cells and 10,000 leucocytes per cubic millimeter, we find as a possible proportion one trypanosome for 2,022,000,000 red cells and 3,370,000 whites. Hence to find the trypanosome in the circulating blood would be merely a piece of good fortune. Further, culture 444, containing 9 c. c. of blood, was negative, yet this amount of blood contains 90,000,000 leucocytes.

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