Unique Morphogenetic And Cell Division Behavior in Populations of Escherichia Coli Treated With 6-Amidinopenicillanic Acid
Unique Morphogenetic And Cell Division Behavior in Populations of Escherichia Coli Treated With 6-Amidinopenicillanic Acid
Blalock, Harvey Antonio
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The rod shape of bacilliform bacteria can be accounted for by only a slight anisotropy in the cellular envelope, resulting in a small preference for longitudinal extension over increases in girth. This property would also account for the increase in diameter found in shifts-up of cells between balanced growth rates. The immediate consequence of such a shift is an increase in the rate of mass synthesis. This cannot be accommodated by the capacity to synthesize envelope without a reduction in sur...face-to-volume ratio, which can only be accomplished by an increase in the diameter of the cell. The new diameter is maintained constant as the relative rates of mass and envelope synthesis are fixed by the growth rate under conditions of balanced exponential growth. This model accounts elegantly for the paradoxical observations that inhibition of division is released by treatment with chloraphcnicol in the cases of temperature-sensitive mutants, or cells treated with nalidixic acid. The Ion mutation is accounted for as providing for a variety of cell envelope having unusually high anisotropy, thus being unable to accommodate physiological conditions which in a normal organism would lead to an increase in diameter.
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