Variation And Carbohydrate Metabolism of Bacilli of the Proteus Group

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+ 1 c. C. Filtered gelatin = liquefaction in 4 days. 8. 50 c. C. +2 % HC1 +1 c. C. Filtered gelatin = no liquefaction in 10 days. All the flasks were tested at the completion of the experiments and pure cultures of P. Vulgaris were found in all of the flasks to which the unfiltered gelatin had been added while those flasks which had re- ceived the filtered gelatin proved sterile. Flasks one and three deve- loped an acid reaction while the reaction of the other flasks remained unchanged. The res...ults are striking and conclusive. In those flasks to which acid had been added or which developed an acid reaction no liquefaction resulted, while in the flasks in which no acid was produced liquefaction took place at a fairly uniform rate. There can be but one interpretation of these results: the liquefaction of the gelatin was inhibited by the acid. We know from the above experiments that dextrose has no effect upon the proteolytic ferment when formed. If to the dextrose gelatin we add in addition to the filtered gelatin a suspension of Proteus vul- garis we get no liquefaction.

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